Ultra-High-Performance Liquid Chromatography (UHPLC)-DAD,ECD,RID

Ultra-High-Performance Liquid Chromatography (UHPLC)-DAD,ECD,RID

Ultra-High-Performance Liquid Chromatography (UHPLC) With all its accessories and Diode Array Detector, Electro-Chemical Detector, and Refractive Index Detector
Inert and biocompatible
Useable up to 5000 Psi.

HPLC Quaternary Pump
Double-piston with high flow constancy and low pulsation, <0.1Mpa
Used as quaternary low-pressure gradient
Optimal for narrow bore chromatography
Built-in system controller with four time-based programmable switches as well as other control signals
Display of pressure profile deviations
Maintenance logbook function
Gradient/concentration precision: ±0.5
Suitable for use in variable temperature: 10 – 30 oC
Flow rate change: 0.001 – 10mL/min

Solvent channel:  4
Flow rate precision: Not more than 0.06% RSD
Voltage requirement: 220±10V, Frequency 50/60Hz

Auto-sampler:
Injection method - Needle-in-flow path
Injection volume accuracy - ±1%
Injection volume reproducibility - RSD: 0.3% maximum
Samples plate–100 or more Sample plate of 1 or 1.5 ml vial
Sample cooler - 4 to 40°C
Voltage requirement: 220±10V, Frequency 50/60Hz
Three different dosing methods: complete loop-filling, partial loop-filling, micro-dosing.
Reproducibility CV< 0.5 % (10 µL)
0.5mL dosing injector, five dosing speeds (adjustment to viscosity)
Injection range 0.1 - 400 µL with standard injector
Electrically operated Rheodyne injection valve
Quick sample mode
Automatic sample vial recognition
Flexible programming functions for sample preparation steps

Oven/ Column compartment:
Suitable to handle column length of 50 – 250mm
Temperature setting range:  4 – 110OC
Voltage requirement: 220±10V, Frequency 50/60Hz

Diode Array Detector:
wavelength range 190-900nm
equipped with both deuterium and tungsten lamp
Noise <2.5 x 10-6AU(250 nm, specified condition)
Drift < 3 x 10-3 AU/h (250 nm, specified condition)
Spectral data library with automatic search facility and spectra comparison.
Subtraction of 3-D background chromatograms
Peak identification
QA and GLP conforming documentation of the analytical results.
Automatic system suitability test including report.
Data transfer in ASCII, AIA, and Excel spread sheet format.
Voltage requirement: 220±10V, Frequency 50/60Hz
Communications:  Local Area Network (LAN), Control Area Network (CAN), RS-232, APG remote, remote, ready, start, and shutdown signals

Safety and maintenance:
Extensive diagnostics,
error detection and display leak detection,
safe leak handling,
leak output signal for shutdown of the pumping system,
low voltage in major maintenance areas

GLP features:  
early maintenance feedback (EMF) for continuous tracking of instrument usage with user-settable limits (purge interval) and feedback messages
electronic records of maintenance and errors,
automated OQ/PV procedures

Refractive Index Detector:
Detection type:  Deflection method
Noise level: ±2.5 x 10-9 RIU/h maximum
Drift:  <1 x 10-7 RIU/h maximum
Refractive index range:  1.00-1.75 RIU
calibrated
Flow cell:  8 - 9µL, 5 bar pressure maximum
Temperature control:  Ambient +5 °C to 60 °C
pH range:  2.0–10.0
Time programmable:  Polarity, peak width
Zero adjustment:  Automatic zero
Valves:  Automatic purge and automatic solvent recycle
Data rate: Up to 37 Hz
Analog output: Recorder/integrator: 100 mV or 1 V, with offset adjustment, RIU range selectable
Voltage requirement: 220±10V, Frequency 50/60Hz
Communications:  Local Area Network (LAN), Control Area Network (CAN), RS-232, APG remote, remote, ready, start and shutdown signals

Safety and Maintenance:
extensive diagnostics,
error detection and display leak detection,
safe leak handling,
leak output signal for shutdown of the pumping system,
low voltage in major maintenance areas

GLP features:  
Early maintenance feedback (EMF) for continuous tracking of instrument usage with user-settable limits (purge interval) and feedback messages
electronic records of maintenance and errors,
automated OQ/PV procedures
The Electrochemical Detector
The Electrochemical Detector must combine reliability, simplicity, and performance for research and development applications.
The electrochemical flow cell must be developed for ultra-trace analysis in analytical, microbore, and capillary scale LC instruments.
The detector must allow DC, pulse and scan modes of operation for analyzing a wide range of compounds.

OPERATING SPECIFICATIONS:
Operating modes Direct Current (DC)
Pulsed Amperometric Detection (PAD) Scan (Available and supported under Empower Software control)
Potential range ± 2000 mV in 10 mV steps (DC, PAD, Scan)
Analog signal output ±1 Volt or ±10 Volt selectable
Output resolution Analog20 bit DAC, 24-bit RS232 digital communication to Empower Software personal workgroup or client computer
Analog signal offset ±50% of Analog signal output, in 10% steps
Auto zero Maximum auto zero determined by Analog signal potential range, triggered by keypad, event in signal, or Empower Software control
Integrated flow cell and column oven 7 °C above ambient to 45 °C, 0.1 °C resolution

DC mode:
Current range 10 nA to 200 µA, 1, 2, 5 sequence steps
Filter time constants 0.1 to 5.0 s in 1, 2, 5 sequence steps
Noise < 2 pA (dummy load 0.47 µF, 300 MW,+800 mV, time constant 1.00 s)
Drift < 8 pA/hour (dummy load 0.47 µF, 300 MW,
+800 mV, time constant 1.00 s) at temperature equal 30 °C

PAD mode:
Range 10 nA to 200 µA, 1, 2, 5 sequence steps
Times t1: 100 to 2000 ms
t2: 100 to 2000 ms
t3 : 0 (off) to 2000 ms in 10 ms steps
Sample times (ts) = 20, 40, 60, 80, 100 ms
Scan mode

Scan range 10 nA to 200 µA in 1, 2, 5 steps
Scan times 1 to 50 mV/s in 1, 2, 5 steps
Scan cycles Half, full, continuous
Method programmability DC mode: nine stored methods total, five stored methods
PAD mode: four stored methods standalone or full Software control
Parameters controlled by Autozero, potential, current range, filter time constants, output offset, flow cell on/off, two output time in standalone mode contact closures and two relay switches
Timed event programming DC and PAD
FLOW CELL
Design Confined wall-jet
Standard flow cell 0.080 µL minimum volume, flow rates from 25.000 µL/min – 2000.000 µL/min
Capillary flow cell 0.011 µL minimum volume, flow rates from 1.000 µL/min – 25.000 µL/min

WORKING ELECTRODE:
Diameters 2.00 and 3.00 mm standard flow cell, 0.75 mm capillary flow cell
Reference electrodes Salt-bridge Ag/AgCl, in-situ Ag/AgCl (ISAAC), Hy-REF
Auxiliary electrode Stainless steel
Spacer thickness 25, 50, and 120 µm
Flow cell dimensions 40 mm diameter, 40 mm length without connectors
Flow cell pressure rating 40 psi
Wetted materials PCTFE, FEP, 316 SS, Viton, Silver, Silver chloride and WE
Working electrodes materials Glassy Carbon, Gold, Platinum, Silver (Copper optional)
Multi HPLC system manager software
Unmatched ease-of-use— the latest generation of software features that has an intuitive dashboard layout with gadgets that guides through every step of the analysis.
The innovative Method Setup Wizard automatically builds methods based on a few simple questions about the application, or from the analysis of a typical sample.
Software that enables the use of remote monitor app to check and control the instrument wherever and whenever wanted.
This allows to simply select the elements of the target to measure and the software shall help pick the appropriate masses based on abundance and potential interferences, and eliminate the need for method development in many applications,
Enable quickly and simultaneously estimate the concentration of all targeted analyte in a sample
Flexible quality control checks – Automate the monitoring of everything from calibration to internal standard responses during a run, ensuring reliable data even during unattended operation.

Acquisition in full-spectrum, peak hopping, and time-resolved modes.
Data analysis that is supported using external and standard calibrations with or without internal standards.
Should support semi-quantitative analysis with a rapid screening of unknowns.
Data archival and retrieval functions.
Data Reporting Programming of customized analysis routines.

System diagnostics software:
21 CFR part 11 compatibility
Offline data analysis

Accessories:
Power cable for each
PTFE filtration frits – standard fit to pump unit and purge valves
Fittings
Needle and needle seat
Auto-sampler gripper and its end cup
Deuterium lamp
Tungsten lamp
Capillary tubes (suitable to connect each component, for waste, for mobile phase pumping)
Ferrules (which fit for all delivered capillary at both ends of each unit)
Finger-tight PEEK fittings
Solvent reservoir, clear screw bottle, with cap(2L capacity graduated colorless bottle (2pcs) with its cup and 2pcs amber type) shall withstand temperature to beck at 300OC
Solvent Inlet Filters
C18 Column Normal and Reversed-phase(150mm *4.6*3.5*µm)
FLD flow cell
HPLC tool kits full set
All necessary gas generators required

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